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Fermentation Strategies for Recombinant Protein Expression in the Methylotrophic Yeast Pichia pastoris 外源蛋白在毕赤酵母中表达策略
Abstract Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as
phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.넶49 21-11-16 -
毕赤酵母额手册-PPIC9K
The Pichia Expression System is based on the yeast Pichia pastoris. Pichia pastoris was
developed into an expression system by scientists at Salk Institute Biotechnology/
Industry Associates (SIBIA) and Phillips Petroleum for high-level expression of
recombinant proteins. All patents for Pichia pastoris and licenses for its use as an
expression system are owned by Research Corporation Technologies (RCT), Inc.,
Tucson, Arizona.넶44 21-09-09